Safety and Efficacy of Percutaneous Injection of Lipogems Micro-Fractured Adipose Tissue for Osteoarthritic Knees
Jay Panchal, MD Gerard Malanga, MD Mitchell Sheinkop, MD
Micro-Fractured Adipose Tissue for Osteoarthritic Knees. Am J Orthop.
Mesenchymal stem cells and regenerative medicine: how Lipogems make them easy, safe and more effective to use
Carlo Tremolada Image institute, Italy
Leggi articoloCorrespondence: Carlo Tremolada, Image institute, Italy,
Email carlo.tremolada@gmail.com
A nonenzymatic and automated closed-cycle process for the isolation of mesenchymal stromal cells in drug delivery applications
Valentina Coccè, 1 Anna Brini, 1, 2 Aldo Bruno Giannì, 1, 3 Valeria Sordi, 4 Angiola Berenzi, 5 Giulio Alessandri, 6 Carlo Tremolada, 7 Silvia Versari, 8 Antonio Bosetto, 9 and Augusto Pessina
Leggi articoloCRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
2
I.R.C.C.S Galeazzi Orthopedic Institute, Milan, Italy
3
Maxillofacial and Dental Unit, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Via Commenda 10, 20122 Milan,
Italy
4
Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy 5
University Research Center “Integrated Models for Prevention and Protection in Environmental and Occupational Health”
(MISTRAL), Brescia, Italy
6
Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, Fondazione IRCCS Neurological Institute C. Besta,
Milan, Italy
7
Image Institute, Milan, Italy
8
Lipogems International Spa, Milan, Italy
9
Hydra srl, Mirandola, Modena, Italy
Micro-fragmented adipose tissue injection associated with arthroscopic procedures in patients with symptomatic knee osteoarthritis
G. Cattaneo1*, A. De Caro1, F. Napoli1, D. Chiapale2, P. Trada2, A. Camera1
Leggi articolo1Spotorno Foundation c/o San Michele Clinics, Viale Pontelungo, 79, 17031 Albenga, SV, Italy
2Orthopaedic Department, San Paolo Hospital, Savona, SV, Italy
*Correspondence: gabrielecattaneo@hotmail.com
Micro‑fragmented adipose tissue injection for the treatment of complex anal fistula: a pilot study accessing safety and feasibility
G. Naldini1, A. Sturiale1, B. Fabiani1, I. Giani1, C. Menconi1
Leggi articolo1Proctological and Perineal Surgery Unit, Cisanello University Hospital, Via Paradisa 2, Pisa, Italy
Mini Review: Mesenchymal Stem Cells and Regenerative Medicine: How Lipogems Technology Make Them Easy, Safe and More Effective to Use
Carlo Tremolada*
Leggi articolo*Corresponding author: Carlo Tremolada, Image institute, Italy, Email: carlo.tremolada@gmail.com
Mesenchymal stem cells: potential for therapy and treatment of chronic non-healing skin wounds
Giovanni Marfia1*,#, Stefania Elena Navone1,#, Clara Di Vito2, Nicola Ughi3, Silvia Tabano4, Monica Miozzo4, Carlo Tremolada5, Gianni Bolla6, Chiara Crotti3, Francesca Ingegnoli3, Paolo Rampini1, Laura Riboni2, Roberta Gualtierotti3, Rolando Campanella1
Leggi articolo1Fondazione IRCCS Ca’Granda Ospedale Maggiore Policlinico; University of Milan; Neurosurgery Unit; Laboratory of Experimental Neurosurgery and Cell Therapy; Milan, Italy
2University of Milan; Department of Medical Biotechnology and Translational Medicine; LITA-Segrate; Milan, Italy
3Division of Rheumatology; Istituto Gaetano Pini; Milan Italy; Department of Clinical Science & Community Health; University of Milan; Milan, Italy
4Fondazione IRCCS C a Granda Ospedale Maggiore Policlinico; University of Milan; Division of Pathology; Milan, Italy
5Istituto Image; Milan, Italy
6Fondazione IRCCS C a Granda Ospedale Maggiore Policlinico; University of Milan; Milan, Italy
*Correspondence to: Giovanni Marfia; Email: giovanni.marfia@unimi.it
#Co-first authors
Menopause: new frontiers in the treatment of urogenital atrophy
G.A. CASAROTTI1, P. CHIODERA2, C. TREMOLADA1
1Image Institute, Milan, Italy
2Department of Pathology, Synlab, Castenedolo (BS), Italy
Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
Pietro Randelli1,2, Alessandra Menon1, Vincenza Ragone1, Pasquale Creo1, Sonia Bergante1, Filippo Randelli1, Laura De Girolamo3, Umberto Alfieri Montrasio3, Giuseppe Banfi3,4, Paolo Cabitza1, Guido Tettamanti1 and Luigi Anastasia12
Leggi articolo1IRCCS Policlinico San Donato, San Donato Milanese, 20097 Milan, Italy
2Department of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy
3IRCCS Istituto Ortopedico Galeazzi, 20161 Milan, Italy
4Universita` Vita-Salute San Ra aele, 20132 Milan, Italy
The Adipose Mesenchymal Stem Cell Secretome Inhibits Inflammatory Responses of Microglia: Evidence for an Involvement of Sphingosine-1-Phosphate Signalling
Giovanni Marfia1,*, Stefania Elena Navone1,*, Loubna Abdel Hadi2,*, Moira Paroni3, Valeria Berno3, Matteo Beretta1, Roberta Gualtierotti4, Francesca Ingegnoli4, Vincenzo Levi2, Monica Miozzo5, Jens Geginat3, Lorenzo Fassina6, Paolo Rampini1, Carlo Tremolada7, Laura Riboni2,† and Rolando Campanella1,† (2016)
Leggi articolo1Laboratory of Experimental Neurosurgery and Cell Therapy, Neurosurgery Unit, Fondazione IRCCS Ca’Granda Ospedale Maggiore Policlinico, University of Milan, Milan, Italy.
2Department of Medical Biotechnology and Translational Medicine, LITA-Segrate, University of Milan, Milan, Italy.
3Istituto Nazionale di Genetica Molecolare ‘‘Romeo ed Enrica Invernizzi,’’ Milan, Italy.
4Division of Rheumatology, Istituto Gaetano Pini, Milano, Italy.
5Division of Pathology, Fondazione IRCCS Ca` Granda Ospedale Maggiore Policlinico, University of Milan, Milan, Italy.
6Department of Health Sciences and Industrial and Information Engineering, University of Pavia, Pavia, Italy.
7Istituto Image, Milan, Italy.
*These authors contributed equally to this work and are considered co-first author
Transnasal endoscopic microfractured fat injection in glottic insufficiency
A. M. Saibene, C. Pipolo, R. Lorusso, S. M. Portaleone and G. Felisati (2015)
Leggi articoloOtolaryngology Department San Paolo Hospital, Department of Health Sciences, University of Milan, Milan, Italy
Lipoaspirate as Autologous Injectable Active Scaffold for One-Step Repair of Cartilage Defects
Michela Bosetti*, Alessia Borrone†, Antonia Follenzi†, Fanuel Messaggio‡, Carlo Tremolada§, and Mario Cannas† (2015)
Leggi articolo*Dipartimento di Scienze del Farmaco, Università del Piemonte Orientale, Novara, Italy.
†Dipartimento di Scienze della Salute, Università del Piemonte Orientale, Novara, Italy.
‡Diabetes Research Institute, University of Miami, Miami, FL, USA.
§IMAGE Institute, Milan, Italy.
The Effect of Intra-articular Injection of Autologous Microfragmented Fat Tissue on Proteoglycan Synthesis in Patients with Knee Osteoarthritis
Damir Hudetz1,2,3,*, Igor Borić1,2,4,5, Eduard Rod1, Željko Jeleč1, Andrej Radić1, Trpimir Vrdoljak1,2, Andrea Skelin1,6, Gordan Lauc6,7, Irena Trbojević-Akmačić6, Mihovil Plečko8, Ozren Polašek4,7, and Dragan Primorac1,3,4,9,10,11,12,* (2017)
Leggi articolo1St. Catherine Specialty Hospital, 49210 Zabok/ 10000 Zagreb, Croatia; igor.boric@svkatarina.hr (I.B.); eduard.rod@svkatarina.hr (E.R.); zeljko.jelec@svkatarina.hr (Z.J.); andrej.radic@svkatarina.hr (A.R.); trpimir.vrdoljak@svkatarina.hr (T.V.); andrea.skelin@svkatarina.hr (A.S.)
2Clinical Hospital “Sveti Duh”, 10000 Zagreb, Croatia
3School of medicine, JJ Strossmayer University of Osijek, 31000 Osijek, Croatia
4School of Medicine, University of Split, 21000 Split, Croatia; ozren.polasek@mefst.hr
5University of Rijeka, Medical School, 51000 Rijeka, Croatia
6Genos Glycoscience Research Laboratory, 10000 Zagreb, Croatia; glauc@genos.hr (G.L.); iakmacic@genos.hr (I.T.-A.)
7Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
8School of Medicine, University of Zagreb, 10000 Zagreb, Croatia; mihovil.plecko@gmail.com
9Gen-info, 10000 Zagreb, Croatia
10Children’s Hospital Srebrnjak, 10000 Zagreb, Croatia
11Eberly College of Science, The Pennsylvania State University, University Park, State College, 16802 PA, USA
12The Henry C. Lee College of Criminal Justice and Forensic Sciences, University of New Haven, West Haven, 06516 CT, USA
*Correspondence: ortohud@gmail.com (D.H.); draganprimorac2@gmail.com (D.P.); Tel.: +385-98-235-623 (D.H.); +385-98-470-710 (D.P.)
Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells
Valentina Ceserani1†, Anna Ferri1*†, Angiola Berenzi2, Anna Benetti2, Emilio Ciusani3, Luisa Pascucci4, Cinzia Bazzucchi4, Valentina Coccè5, Arianna Bonomi5, Augusto Pessina5, Erica Ghezzi6, Offer Zeira6, Piero Ceccarelli4, Silvia Versari7, Carlo Tremolada7 and Giulio Alessandri1 (2017)
Leggi articolo1Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, IRCCS Neurological Institute C. Besta, Via Celoria 11, 20131 Milan, Italy
2Department of Clinical and Experimental Sciences, Institute of Pathological Anatomy, University of Brescia, Brescia, Italy
3Laboratory of Clinical Pathology and Neurogenetic Medicine, Fondazione IRCCS Neurological Institute C. Besta, Milan, Italy.
4Department of Veterinary Medicine, University of Perugia, Perugia, Italy
5Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
6San Michele Veterinary Hospital, Tavezzano con Villavesco, Lodi, Italy
7Image Institute, Milan, Italy
†Equal contributors
*Correspondence: anna.ferri@istituto-besta.it
Autologous and micro-fragmented adipose tissue for the treatment of diffuse degenerative knee osteoarthritis
A. Russo1, V. Condello1, V. Madonna1, M. Guerriero2, C. Zorz1 (2017)
Leggi articolo1Orthopaedic Department, Sacro Cuore – Don Calabria Hospital, Via Don A. Sempreboni, 5, 37024 Negrar, VR, Italy
2Computer Science Department, University of Verona, Verona, VR, Italy
Non-Responding Knee Pain with Osteoarthritis, Meniscus and Ligament Tears Treated with Ultrasound Guided Autologous, Micro-Fragmented and Minimally Manipulated Adipose Tissue
Richard David Striano1, Valeria Battista2, Norma Bilboo1 (2017)
Leggi articolo1Optimum Joint, New York, USA
2Istituto Image, Milano, Italy
Microfractured and Purified Adipose Tissue (LipogemsTM system) Injections forTreatment of Atrophic Vaginitis
Jennifer Fantasia1, Halland Chen2, Janice A. Santos Cortes1 (2016)
Leggi articolo1Department of Urology, Brown University, USA
2Department of Regenerative Medicine, Brown University, USA
Low back pain treated with disc decompression and autologous micro-fragmented adipose tissue: a case report
P. Grossi1, S. Giarratana2, S. Cernei3, S. Grossi4, F.M. Doniselli5
Leggi articoloCellR4 2016; 4 (1): e1772
1O.U. Regional Anesthesia and Pain Treatment, IRCCS Policlinico San Donato, Milan, Italy
2Faculty of Medicine, University of Milan, Milan, Italy
3School of Specialization in Anesthesiology, University Amadeo Avogadro, Novara, Italy
4School of Specialization in Orthopedics and Traumatology, University of Pisa, Pisa, Italy
5School of Specialization in Radiology, University of Milan, Milan, Italy
Injection of autologous micro-fragmented adipose tissue for the treatment of post-traumatic degenerative lesion of knee cartilage: a case report
M. Franceschini, C. Castellaneta, G. Mineo
Leggi articoloCellR4 2016; 4 (1): e1768
Orthopaedic and Trauma Department, Istituto Ortopedico “G. Pini”, University of Milan, Milan, Italy
Microfractured Lipoaspirate May Help Oral Bone and Soft Tissue Regeneration: a Case Report
R. Benzi1, G. Marfia3, M. Bosetti4, G. Beltrami2, A.S. Magri2, S. Versari2, C. Tremolada2 (2016)
Leggi articolo1Benzi Dental Clinic, Vigevano (PV), Italy
2Image Institute, Milan, Italy
3Laboratory of Experimental Neurosurgery and Cell Therapy, Neurosurgery Unit, Fondazione IRCCS, CA’ Granda Ospedale Policlinico, Milan, Italy
4Human Anatomy, Scienze del Farmaco Department, UPO, Novara, Italy
Adipose Tissue and Mesenchymal Stem Cells: State of the Art and Lipogems® Technology Development
C. Tremolada*, V. Colombo*, C. Ventura** (2016)
Leggi articolo*Image Institute, Milan, Italy
**Stem Wave Institute for Tissue Healing (SWITH)—Ettore Sansavini Health Science Foundation, Lugo, Ravenna, Italy
The Use of Physical Energy for Tissue Healing
C. Ventura*, F. Bianchi, C. Cavallini, E. Olivi, R. Tassinari
Leggi articoloEuropean Heart Journal Supplements (2015)17: A69-A73
*Stem Wave Institute for Tissue Healing (SWITH), E. Sansavini Health Science Foundation, Ravenna, Italy
Non-Responsive Knee Pain with Osteoarthritis and Concurrent Meniscal Disease Treated With Autologous Micro-Fragmented Adipose Tissue Under Continuous Ultrasound Guidance
R.D. Striano, H. Chen, N. Bilbool, K. Azatullah, J. Hilado, K. Horan
Leggi articoloCellR4 2015; 3 (5): e1690
Regenerative Medicine, Optimum Joint, New York, USA
Recovery of Function in Anal Incontinence after Micro-Fragmented Fat Graft (Lipogems®) Injection: Two Years Follow Up of the First 5 Cases.
A. Giori*, C. Tremolada, R. Vailati, S.E. Navone, G. Marfia, A.I. Caplan
Leggi articoloCellR4 (2015), 3 (2): e1544
*Department of General Surgery, San Paolo University Hospital, Milan, Italy
Adipose Mesenchymal Stem Cells and “Regenerative Adipose Tissue Graft” (Lipogems®TM) for Musculoskeletal Regeneration
C. Tremolada*, G. Beltrami, A. Magri, F. Bianchi, C. Ventura, C. Di Vito, R. Campanella, S.E. Navone, G. Marfia And A.I. Caplan
Leggi articoloEuropean Journal of Musculoskeletal Diseases (2014), Vol. 3, no. 2
*Image Institute, Milan, Italy
Human Stem Cell Exposure to Developmental Stage Zebrafish Extracts: a Novel Strategy for Tuning Stemness and Senescence Patterning
S. Canaider*, M. Maioli, F. Facchin, E. Bianconi, S. Santaniello, G. Pigliaru, L. Ljungberg, F. Burigana, F. Bianchi, E. Olivi, C. Tremolada, P. M. Biava and C. Ventura
Leggi articoloCellR4 (2014) 2(5), e1226
*Department of Specialistic, Diagnostic, and Experimental Medicine, University of Bologna, Italy
Fair or Foul: Time for Standard Protocols for Potential Application of Adipose-Derived Stem Cells
J Stem Cell Res Ther (2014), 4(220):2
* Department of General, Visceral und Vascular Surgery, Otto-von-Guericke University, Germany
Radioelectric Asymmetric Conveyed Fields and Human Adipose-Derived Stem Cells Obtained With a Nonenzymatic Method and Device: A Novel Approach to Multipotency
Cell transplantation (2014), 23(12): 1489-1500
*Department of Biomedical Sciences, University of Sassari, Sassari, Italy
†Laboratory of Molecular Biology and Stem Cell Engineering – National Institute of Biostructures and Biosystems, Bologna, Italy
‡Department of Regenerative Medicine, Rinaldi Fontani Institute, Florence, Italy
Differences in exosome content of human adipose tissue processed by non-enzymatic and enzymatic methods
CellR4 (2014), 3 (1): e1423
* Diabetes Research Ins”tute,University ofMiami,Miller School ofMedicine,Miami, FL,USA
Case report Intersphincteric anal lipofilling with micro-fragmented fat tissue for the treatment of faecal incontinence: preliminary results of three patients
Videosurgery and Other Miniinvasive Techniques (2014), DOI: 10.5114/wiitm.2014.47435
*Department of General and Mininvasive Surgery University of Naples Federico II, Napoli, Italy
Lipogems®, a New Modality of Fat Tissue Handling to Enhance Tissue Repair in Chronic Hind Limb Ischemia
CellR4 (2014) 2 (6): e1289
*Stem Wave Institute for Tissue Healing (SWITH), E. Sansavini Health Science Foundation, Ravenna, Italy
Characteristics and Properties of Mesenchymal Stem Cells Derived from Micro-fragmented Adipose Tissue.
Cell Transplant. 2014 April 29. [Accepted for pubblication].
*Laboratorio di Farmacologia, Dipartimento di Scienze della Salute, Università degli Studi di Milano.
Micro Fractured and Purified Adipose Tissue Graft (Lipogems®) Can Improve the Orthognathic Surgery Outcomes Both Aesthetically and in Postoperative Healing
CellR4 2014; 2 (4): e1118. 2014 July 28
Ricerca di nuove strategie differenziative: analisi degli effetti di stimoli fisici e molecolari su cellule mesenchimali umane isolate da tessuto adiposo
Dottorato di Ricerca in Scienze Farmacologiche, Tossicologiche, dello Sviluppo e Movimento Umano.
*Alma Mater Studiorum – University of Bologna, Italy
Mesenchymal Stem Cells from human adipose tissue: Characterization and Immunomodulatory Properties of Tissue and Cellular Products
Dottorato di Ricerca Fisiopatologia, Farmacologia, Clinica e Terapia delle Malattie Metaboliche. University of Milan, Italy.
Adipose-derived stem cells and tissue revascularization: enhancing islet survival and performance for diabetes care
Dottorato Di Ricerca In Scienze Chirurgiche. Alma Mater Studiorum – University of Bologna, Italy
A new nonenzymatic method and device to obtain a fat tissue derivative highly enriched in pericyte- like elements by mild mechanical forces from human lipoaspirate
Cell transplantation (2013), 22(11): 2063-2077
*Laboratory of Molecular Biology and Stem Cell Engineering-National Institute of Biostructures and Biosystems, Bologna, Italy
Preparation and method for producing a preparation comprising mesenchymal stem cells
Tremolada C (2013) – US Patent App. 14/382,298
Leggi articoloRadio electric asymmetric conveyed fields and human adipose-derived cells obtained with a non-enzymatic method and device: a novel approach to multipotency
Cell Transplant. 2013 Aug 30. doi: 10.3727/096368913X672037. [Epub ahead of print]
*Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems, Bologna, Italy
A new nonenzymatic method and device to obtain a fat tissue derivative highly enriched in pericyte-like elements by mild mechanical forces from human lipoaspirates
Cell Transplant. 2013;22(11):2063-77. doi: 10.3727/096368912X657855. Epub 2012 Oct
*Laboratory of Molecular Biology and Stem Cell Engineering-National Institute of Biostructures and Biosystems, Bologna, Italy.
Adipocyte transplantation and stem cells: plastic surgery meets regenerative medicine.
Cell Transplant. 2010;19(10):1217-23. doi: 10.3727/096368910X507187. Epub 2010 May 4.
*Istituto Image and San Paolo Hospital, Milan, Italy