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T*
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[(fective)-426.1(preparation)-424.7(method)-418.1(would)-421.2(induce)-423.8(the)-419.3(highest)-423.6(ana-)]TJ
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T*
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[(bolic)-306.4(balance)-306.2(of)-307.4(ECMsuggestive)-309.9(of)-301.7(regenerative)-313(capacity)]TJ
T*
[(in)-261.5(cartilageas)-262.2(well)-260.1(as)-256.6(to)-255.8(provide)-262.4(rationale)-255.7(for)-262.4(the)-258(selection)]TJ
T*
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[(knee)-340.8(OA)-332.2(pathologies.)]TJ
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[(Metho)-13.1(ds)]TJ
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[(Level)-337.5(of)-330.5(Evidence:)-343.2(Level)-331.7(1,)-332.8(Therapeutic)-340.7(Study)]TJ
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[(Sta)-9(tement)-340.5(of)-333.6(institutio)-11.7(nal)-333.9(regulat)-10.1(ory)-333.5(appro)-8.3(val)]TJ
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[(tained)-625.5(from)-627.6(full-thickness)-628.3(skin)-620.6(samples)-627.3(discarded)-629.6(from)]TJ
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[(standard)-581.2(of)-578.1(care)-578.1(panniculectomy)-580.4(procedures)-584.5(under)-576.9(Uni-)]TJ
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[(versity)-319.9(of)-319(Pittsburgh)-322(IRB)-316.4(exemption)-324.8(#0511186XM.)-315.7(Human)]TJ
T*
[(chondrocyte)-372.8(lines)-369.6(were)-370.4(donated)-367.3(by)-370.8(Dr.)-363.6(Peter)-370.6(Alexander)-371.5(as)]TJ
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[(frozen)-556(cryovials)-547.9(for)-550.4(which)-554.8(patient)-551.8(consent)-551.4(and)-545.9(protocol)]TJ
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[(approval)-276.2(were)-278.3(obtained)-276.3(from)-276.3(the)-275.3(University)-272.4(of)-272.9(Pittsburgh)-276(as)]TJ
T*
[(previously)-340.5(published)-333.7([18].)]TJ
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[(Chon)-10.3(drocyte)-337.8(cell)-338.7(culture)]TJ
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[(Mildly)-267.1(degenerative)-266.9(articular)-267.2(chondrocytes)-264.3(were)-261(obtained)]TJ
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[(and)-390.4(puried)-383.2(separately)-390.1(from)-391.5(two)-384.4(de-identied)-391.3(donors)-386.5(who)]TJ
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[(underwent)-289.7(total)-283.5(knee)-289(arthroplasty)-286.4(\(a)-285.5(37-year-old)-285.2(male)-290.3(and)-280.9(a)]TJ
T*
[(47-year-old)-452.2(female,)-452.6(Mankin)-446.5(score)-451.1(36\).)-445.5(These)-446.6(cells)-451.4(were)]TJ
0 -1.1174 TD
[(plated)-827.1(from)-817.7(frozen)-821(cryovials)-824.4(and)-822.3(expanded)-824.4(in)-820.2(two-)]TJ
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T*
[(ambient)-322.6(oxygen)-324.6(\(20%\))-317.5(and)-321.2(carbon)-325.7(dioxide)-320.2(\(5%\))-322.2(conditions)]TJ
T*
[(and)-304(using)-299.2(DMEM/F12)-306.4(media)-302.9(\(Life)-301.7(Technologies,)-303.6(Carlsbad,)]TJ
0 -1.1174 TD
[(CA\),)-296.6(with)-287.8(media)-297.2(changed)-293.4(every)-290(2)-289.1(days.)-296.8(Once)-289.8(near)-294.7(full)-292.6(con-)]TJ
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[(uency)-429.2(\(90%\),)-430.4(cells)-422.6(were)-433.8(then)-426.2(put)-423.9(into)-425.1(three-dimensional)]TJ
T*
[(culture)-309.8(at)-303(a)-301.8(density)-308.4(of)-301.7(4)]TJ
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()Tj
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(6)Tj
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[(cells/mL)-307.5(for)-302.7(48)-169.2(h)-300.6(through)-307.3(use)]TJ
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[(native)-625.5(chondrocyte)-620.5(phenotype)-622.9([19].)-618.3(These)-619.4(chondrocyte-)]TJ
T*
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[(scribe)-8.8(d)-329.4(hereunder.)]TJ
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[(pro)-8.4(cessing)]TJ
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(n)Tj
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(=)Tj
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(6\))Tj
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[(Stromal)-344.2(vascular)-348.8(fraction.)]TJ
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[(SVF)-343.7(was)-339.2(obtained)-345.5(through)-341.9(en-)]TJ
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/F6 1 Tf
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(2)Tj
47.8897 0 TD
[(KOKAI)-331.2(ET)-338.7(AL.)]TJ
ET
endstream
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[(salt)-645(solution)-636.8(\(HBSS;)-641.9(Lonza,)-640.3(Basel,)-645.8(Switzerland\).)-643.2(After)]TJ
T*
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(Nanofat.)Tj
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T*
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[(Bead-mill)-357.2(processing.)]TJ
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[(Another)-349.7(form)-351.1(of)-353.5(lipoaspirate)-356.8(prep-)]TJ
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T*
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T*
[(Per)-747.3(manufacturers)-746.8(instructions,)-749.4(tissue)-744.9(fragments)-750.6(were)]TJ
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[(placed)-338.5(in)-336.4(the)-338.7(provided)-338.4(lter)-335(shaker)-342.8(with)-333.8(mechanical)-340.9(beads,)]TJ
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[(followed)-466.1(by)-457.2(saline)-458.3(wash)-461.3(in)-457.3(dialysis)-459.2(tubing.)-459.6(After)-462.6(harvest)]TJ
T*
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[(immediately)-341.1(placed)-338.5(in)-330.6(co-culture)-337.2(with)-333.8(chondrocytes.)]TJ
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9.9626 0 0 9.9626 59.7543 505.9841 Tm
[(Chon)-10.3(drocyte-ad)-10.4(ipose)-334.7(co)-6.4(-culture)-338(system)]TJ
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9.843 0 0 9.843 69.7323 488.1259 Tm
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T*
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[(eit)12.6(her)-344.3(36)-347.8(or)-342(72)-157.7(h.)-350.1(Bu)14(oya)12.8(nt)-347.9(adi)12.7(pos)14.2(e)-347.8(tis)13.9(sue)-344.1(pre)12.9(par)12.8(ati)12.6(on)15.1(s)-352.2(wer)12(e)]TJ
25.5097 27.422 TD
[(hel)12.7(d)-283.3(i)0(n)-284.6(the)-281.1(tra)12.7(nsw)13.3(el)13.8(l)-289.2(ins)14(ert)12.7(s)-288.9(and)-275.2(sub)14.2(mer)11.6(ged)-280.9(in)-278.8(cul)12.7(tur)13.9(e)-290.2(med)11.6(ia)]TJ
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[(thr)13.9(oug)14(h)-404.3(use)-395.9(of)-399.6(a)-411.2(t)0(w)14.1(o-p)14(art)-402(pr)15.1(ess)13.2(-t)-400.7(dev)12.8(ic)13.8(e)-411.2(cus)13(to)15(m)-411.3(m)0(a)12.7(d)0(e)-400.8(i)0(n)]TJ
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[(Sol)14(idW)17.3(ork)14(s)-311.9(CAD)11.2(/CA)11.9(M)-313(\(Da)12(ssa)13.2(ult)-303.1(Sys)14.3(tem)17.2(s,)-309.6(Wal)10.4(tha)12.7(m,)-305.3(MA\))12.2(.)]TJ
T*
[(Pre)13(ss-)14.4(t)-480.2(dev)12.8(ic)13.8(es)-487(we)13(re)-481.4(3D)-481.1(pri)13.9(nte)12.7(d)-484.9(usi)14(ng)-480.3(bio)13.8(log)13.9(ica)11.5(lly)-475.9(ine)18.4(rt)]TJ
T*
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[(MI\))-188.4(and)-194.5(he)13.9(ld)-198.2(a)-198.1(met)11.4(al)-193.6(scr)13(een)-195.7(\(0.)17.4(006)14(-in)13.9(ch)-193.4(por)14(e)-203.8(siz)12.9(e;)-193.6(McM)11(ast)12.9(er)13.9(-)]TJ
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T*
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[(RT-PCR)-341.5(analysi)-9.3(s)]TJ
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[(After)-537.5(co-culture,)-536.5(total)-537(mRNA)-538.3(was)-535(extracted)-544.7(from)-535.5(the)]TJ
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[(chondrocytes)-350.7(and)-344.3(adipos)-7.7(e)-342.1(tissues)-354.1(following)-345.2(methods)-349.9(estab-)]TJ
T*
[(lished)-353.4(in)-359.4(previous)-355.5(studies)-360.1([20,21].)-353.2(RT-PCR)-362.1(was)-356.5(performed)]TJ
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[(on)-284.4(isolated)-293.6(mRNA)-290.6(from)-287.8(the)-286.8(chondrocytes)-293.1(and)-286.7(from)-287.8(adipos)-7.7(e)]TJ
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[(tissues)-423.2(using)-425.9(the)-419.3(comparative)]TJ
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(DD)Tj
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[(Ct)-423.8(method)-423.8(using)-420.1(glyce)-8(r-)]TJ
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