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Exosomes (EXOs) aremembrane vesicles released bymost of cell types. EXOs contain lipids, proteins, and RNA (mRNAs, micro RNAs, non-coding RNAs). EXOs have been shown to have diagnostic and therapeutic potential. Secreted EXOs transport to other cells and deliver their contents into recipient cells playing a role in cell-to-cell communication. Adipose tissue and adipose-derived stromal cells (ADSCs) represent a promising therapeutic option in regenerative medicine and tissue engineering applications. ADSCs effects on immunomodulation and tissue repair may be attributed to paracrine secreted factors, including EXOs. Here we examine whether human adipose tissue processing methods could affect the EXOs content of the final cell/tissue, comparing non-enzymatic and enzymatic methods.